{"id":557,"date":"2024-04-29T01:21:38","date_gmt":"2024-04-29T01:21:38","guid":{"rendered":"https:\/\/src.cikeys.com\/2024\/?page_id=557"},"modified":"2024-04-29T01:21:39","modified_gmt":"2024-04-29T01:21:39","slug":"restoring-pluripotency-of-p19-cl6-stem-cells-via-5-azacytidine-and-1-dmso","status":"publish","type":"page","link":"https:\/\/src.cikeys.com\/2024\/conference-schedule\/poster-presentations\/poster-presentations-session-3\/restoring-pluripotency-of-p19-cl6-stem-cells-via-5-azacytidine-and-1-dmso\/","title":{"rendered":"Restoring Pluripotency of P19.CL6 Stem Cells via 5′-azacytidine and 1% DMSO"},"content":{"rendered":"\n

Sonya Sahar Khiz Langroody, Chiarra Limos, Aryan Mishor, Tristan Valencia, Kyle Butac, & Dr. James Harber<\/h2>\n\n\n\n
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There are unique types of cells that give rise to many different types of cells and tissues in the body that are called pluripotent stem cells like P19.CL6 that are able to differentiate into three tissue types of mesoderm, ectoderm, and endoderm. They\u2019ve the ability to undergo self-renewal and differentiation making them valuable for scientific research and beneficial for science and therapeutic purposes. It is difficult to maintain and increase pluripotency stem cells and their undergoing self-renewal due to their overgrowth of fibroblasts (ectoderm) per passage and maintaining their cell culture conditions. As a result of this, reversing methylation and renewing naive epigenetic expression by restoring the less specialized state of cells has been accomplished via using 5\u2019-azacytidine (5\u2019-aza) on P19.CL6. The objective of our experiment is to give rise to more pluripotent stem cells generated via using 5\u2019-aza to demethylate their DNA possessing the capacity to give rise to the three tissue types.\u00a0 Our hypothesis is that the 5\u2019-aza treatment would enhance and increase the restoration of more pluripotent cells that would promote the differentiation of mesoderm, ectoderm, and endoderm. In this experiment, embryoid bodies (EBs) grew on ultra-low attachment plates that were treated with 10 \u00b5m of 5\u2019-aza within three days and then switched to mesoderm (cardiac) facilitating differentiation media with 1% of DMSO for seven days which were placed in a coated gelatin plate. The media was changed twice a week and the FBS concentration on day seven was reduced from 10% to 5% for better growth of cells. After fourteen days, we witnessed black pigmented cells in all of our two six well dishes, signifying retinal pigment epithelium, RPE, creating an ectoderm layer. At twenty-one days, beating next to the black cells of RPE were seen resulting in the growth of the mesoderm layer. Nerve cells have been noted throughout the plates indicating an endoderm tissue layer growth. There have been numerous sites of other cells that resemble The shape of the bunny throughout the two plates that are heart cells. In conclusion, 5\u2019-aza shows a promising and effective method for demethylation and pluripotent restoration.<\/p>\n<\/div>\n\n\n\n

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Poster Presentation<\/strong><\/a><\/p>\n\n\n\n

Session 3<\/a><\/p>\n\n\n\n

2:45pm \u2013<\/strong> 4:00pm
Grand Salon<\/p>\n\n\n\n

Biology<\/strong><\/p>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

Sonya Sahar Khiz Langroody, Chiarra Limos, Aryan Mishor, Tristan Valencia, Kyle Butac, & Dr. James Harber There are unique types of cells that give rise to many different types of cells and tissues in the body that are called pluripotent stem cells like P19.CL6 that are able to differentiate into three tissue types of mesoderm, […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":48,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"categories":[5,8],"tags":[26],"class_list":["post-557","page","type-page","status-publish","hentry","category-poster-presentations","category-session-3","tag-biology","post"],"_links":{"self":[{"href":"https:\/\/src.cikeys.com\/2024\/wp-json\/wp\/v2\/pages\/557","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/src.cikeys.com\/2024\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/src.cikeys.com\/2024\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/src.cikeys.com\/2024\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/src.cikeys.com\/2024\/wp-json\/wp\/v2\/comments?post=557"}],"version-history":[{"count":1,"href":"https:\/\/src.cikeys.com\/2024\/wp-json\/wp\/v2\/pages\/557\/revisions"}],"predecessor-version":[{"id":640,"href":"https:\/\/src.cikeys.com\/2024\/wp-json\/wp\/v2\/pages\/557\/revisions\/640"}],"up":[{"embeddable":true,"href":"https:\/\/src.cikeys.com\/2024\/wp-json\/wp\/v2\/pages\/48"}],"wp:attachment":[{"href":"https:\/\/src.cikeys.com\/2024\/wp-json\/wp\/v2\/media?parent=557"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/src.cikeys.com\/2024\/wp-json\/wp\/v2\/categories?post=557"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/src.cikeys.com\/2024\/wp-json\/wp\/v2\/tags?post=557"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}