{"id":377,"date":"2023-05-06T02:39:25","date_gmt":"2023-05-06T02:39:25","guid":{"rendered":"http:\/\/src.cikeys.com\/2023\/?page_id=377"},"modified":"2023-05-06T03:07:17","modified_gmt":"2023-05-06T03:07:17","slug":"get-dry-or-die-trying-molecular-and-chemical-chaperones-are-essential-for-desiccation-tolerance","status":"publish","type":"page","link":"https:\/\/src.cikeys.com\/2023\/conference-schedule\/poster-presentations-session-1\/get-dry-or-die-trying-molecular-and-chemical-chaperones-are-essential-for-desiccation-tolerance\/","title":{"rendered":"Get Dry or Die Trying: Molecular and Chemical Chaperones are Essential for Desiccation Tolerance"},"content":{"rendered":"\n<h2 class=\"wp-block-heading\">Alisha Greenlaw, Emily Heltsley, &amp; Dr. Hugo Tapia<\/h2>\n\n\n\n<!--more-->\n\n\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-7387b849 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:66.66%\">\n<h3 class=\"wp-block-heading\">Abstract<\/h3>\n\n\n\n<p class=\"wp-block-paragraph\">Most cell\u2019s chemical processes involve water, making the loss of it a severe stressor to homeostasis. Anhydrobiotic organisms can tolerate desiccation for extended periods. Understanding the molecular basis for their desiccation tolerance may provide novel insights into stress biology and critical tools for engineering drought-tolerant crops. Using the anhydrobiote, Saccharomyces cerevisae, we show that trehalose and the chaperones Hsp12 and Hsp104, synergize to mitigate the inviability caused by the lethal stresses of desiccation. Interestingly, the protein chaperone Hsp104 compensates for loss of trehalose during short-term, but not long-term, desiccation. We will extend our long-term studies by coupling limited humidity while drying. We propose that desiccated cells initially accumulate both protein and chemical chaperones, like Hsp104 and trehalose, respectively. As desiccation extends, the activities of the protein chaperones are lost because of their complexity and requirement for energy, leaving trehalose as the major protector against the aggregation of cytoplasmic and membrane proteins.<\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:33.33%\">\n<h3 class=\"wp-block-heading\">Details<\/h3>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>Session 1<\/strong><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>9:30am \u2013 11:00am<\/strong><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>Grand Salon<\/strong><\/p>\n<\/div>\n<\/div>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n\n\n\n<div class=\"wp-block-comments\">\n\n\n\n\n\n\t<div id=\"respond\" class=\"comment-respond wp-block-post-comments-form\">\n\t\t<h3 id=\"reply-title\" class=\"comment-reply-title\">Leave a Reply <small><a rel=\"nofollow\" id=\"cancel-comment-reply-link\" href=\"\/2023\/wp-json\/wp\/v2\/pages\/377#respond\" style=\"display:none;\">Cancel reply<\/a><\/small><\/h3><form action=\"https:\/\/src.cikeys.com\/2023\/wp-comments-post.php\" method=\"post\" id=\"commentform\" class=\"comment-form\"><p class=\"comment-notes\"><span id=\"email-notes\">Your email address will not be published.<\/span> <span class=\"required-field-message\">Required fields are marked <span class=\"required\">*<\/span><\/span><\/p><p class=\"comment-form-comment\"><label for=\"comment\">Comment <span class=\"required\">*<\/span><\/label> <textarea id=\"comment\" name=\"comment\" cols=\"45\" rows=\"8\" maxlength=\"65525\" required><\/textarea><\/p><p class=\"comment-form-author\"><label for=\"author\">Name <span class=\"required\">*<\/span><\/label> <input id=\"author\" name=\"author\" type=\"text\" value=\"\" size=\"30\" maxlength=\"245\" autocomplete=\"name\" required \/><\/p>\n<p class=\"comment-form-email\"><label for=\"email\">Email <span class=\"required\">*<\/span><\/label> <input id=\"email\" name=\"email\" type=\"email\" value=\"\" size=\"30\" maxlength=\"100\" aria-describedby=\"email-notes\" autocomplete=\"email\" required \/><\/p>\n<p class=\"comment-form-url\"><label for=\"url\">Website<\/label> <input id=\"url\" name=\"url\" type=\"url\" value=\"\" size=\"30\" maxlength=\"200\" autocomplete=\"url\" \/><\/p>\n<p class=\"comment-form-cookies-consent\"><input id=\"wp-comment-cookies-consent\" name=\"wp-comment-cookies-consent\" type=\"checkbox\" value=\"yes\" \/> <label for=\"wp-comment-cookies-consent\">Save my name, email, and website in this browser for the next time I comment.<\/label><\/p>\n<p class=\"form-submit\"><input name=\"submit\" type=\"submit\" id=\"submit\" class=\"submit\" value=\"Post Comment\" \/> <input type='hidden' name='comment_post_ID' value='377' id='comment_post_ID' \/>\n<input type='hidden' name='comment_parent' id='comment_parent' value='0' \/>\n<\/p><p style=\"display: none !important;\" class=\"akismet-fields-container\" data-prefix=\"ak_\"><label>&#916;<textarea name=\"ak_hp_textarea\" cols=\"45\" rows=\"8\" maxlength=\"100\"><\/textarea><\/label><input type=\"hidden\" id=\"ak_js_1\" name=\"ak_js\" value=\"53\"\/><script>\ndocument.getElementById( \"ak_js_1\" ).setAttribute( \"value\", ( new Date() ).getTime() );\n<\/script>\n<\/p><\/form>\t<\/div><!-- #respond -->\n\t<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Alisha Greenlaw, Emily Heltsley, &amp; Dr. Hugo Tapia<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":42,"menu_order":0,"comment_status":"open","ping_status":"closed","template":"","meta":{"ngg_post_thumbnail":0,"footnotes":""},"categories":[5,3,9],"tags":[21],"class_list":["post-377","page","type-page","status-publish","hentry","category-poster","category-presentations","category-session-1-poster","tag-biology"],"_links":{"self":[{"href":"https:\/\/src.cikeys.com\/2023\/wp-json\/wp\/v2\/pages\/377","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/src.cikeys.com\/2023\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/src.cikeys.com\/2023\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/src.cikeys.com\/2023\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/src.cikeys.com\/2023\/wp-json\/wp\/v2\/comments?post=377"}],"version-history":[{"count":1,"href":"https:\/\/src.cikeys.com\/2023\/wp-json\/wp\/v2\/pages\/377\/revisions"}],"predecessor-version":[{"id":438,"href":"https:\/\/src.cikeys.com\/2023\/wp-json\/wp\/v2\/pages\/377\/revisions\/438"}],"up":[{"embeddable":true,"href":"https:\/\/src.cikeys.com\/2023\/wp-json\/wp\/v2\/pages\/42"}],"wp:attachment":[{"href":"https:\/\/src.cikeys.com\/2023\/wp-json\/wp\/v2\/media?parent=377"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/src.cikeys.com\/2023\/wp-json\/wp\/v2\/categories?post=377"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/src.cikeys.com\/2023\/wp-json\/wp\/v2\/tags?post=377"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}